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Image Search Results
Journal: Neuroscience research
Article Title: Whole-brain long-range connectivity of glutamatergic, GABAergic, parvalbumin-expressing and somatostatin-expressing neurons in mouse somatosensory cortex.
doi: 10.1016/j.neures.2025.104912
Figure Lengend Snippet: Fig. 1. Tracing of whole-brain inputs and outputs of glutamatergic and GABAergic neurons in the SSp. (A) Whole-brain input and output tracing of glutamatergic, GABAergic, PV+ , and SOM+ neurons in the SSp. (B) Representative coronal sections showing the injection site (Imaged using a 10x objective on a confocal mi croscope (Leica, DMi8)), scale bar, 1 mm. The middle and right panel shows an enlarged view of the white box (Imaged using a 20x objective on a confocal mi croscope (Leica, DMi8)). Green: cells labeled by CVS-EnvA-ΔG-EGFP. Red: cells labeled by rAAV-EF1α-DIO-mCherry-TVA. Yellow: starter cells. Scale bar, 50 μm. (C) Relationship between starter cells and long-range input neurons. Fourteen mice were used for analysis, with 3 Vglut2-Cre and SOM-Cre mice, and 4 Gad2-Cre and PV- Cre mice, respectively. (D) Representative coronal sections showing the distribution of monosynaptic input neurons to SSp glutamatergic neurons. Scale bars,1 mm (top) and 50 μm (bottom). (E) Representative coronal sections showing the distribution of axon projections from SSp glutamatergic neurons. Scale bars, 1 mm (top) and 50 μm (bottom).
Article Snippet: Star★Methods KEY RESOURCES TABLE Reagent or Resource Source Identifier/Product number/ version Additional information Ethylurethane Sinopharm Chemical Reagent Co., Ltd 30191218 Phosphate buffer solution (PBS) Sigma-Aldrich P3813 Paraformaldehyde (PFA) Sinopharm Chemical Reagent Co., Ltd 80096618 Lowicryl HM20 resin Polysciences, Inc 15924–1 Agarose Sigma-Aldrich A0576 Sodium periodate Sigma-Aldrich 311448 Agarose Sigma-Aldrich A0576 Sodium periodate (NaIO4) Sigma-Aldrich 311448 Vglut2-ires-Cre The Jackson Laboratory RRID: IMSR_JAX:028863 Gad2-IRES-Cre The Jackson Laboratory RRID: IMSR_JAX:010802 Pvalb-IRES-Cre (PVCre) The Jackson Laboratory RRID: IMSR_JAX:017320 (continued on next column) (continued ) Sst-IRES-Cre (SOMCre) The Jackson Laboratory RRID: IMSR_JAX:013044 C57BL/6 J Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) RRID: IMSR_JAX:000664 rAAV-EF1α-DIOmCherry-F2A-TVA (AAV/9) BrainVTA Co., Ltd. BC− 0061 ≥ 2.00E+ 12 vg/ml rAAV-EF1α-DION2cG (AAV/9) BrainVTA Co., Ltd. BC− 0442 ≥ 2.00E+ 12 vg/ml CVS-EnvA-ΔG-EGFP BrainVTA Co., Ltd. BC-RV-CVSEnvA461 ≥ 1.00E+ 8 IFU/ml rAAV-EF1α-DIOEGFP (AAV/9) BrainVTA Co., Ltd. BC− 0015 ≥ 2.00E+ 12 vg/ml Digital Stereotaxic Instruments RWD Life Science 68803 Mouse Adaptors RWD Life Science 68030 Quintessential Stereotaxic Injector (QSI) Stoelting Company 53311 Fully automated vibrating
Techniques: Injection, Labeling